A Simple Micropreparative Gel Electrophoresis Technique for Purification of Nanoscale Materials
نویسندگان
چکیده
Many biochemical, biomedical, and material applications hinge on the ability to effectively separate purify nanoscale materials. Though this need is largely addressed with biological macromolecules using a variety of chromatographic electrophoretic purification techniques, such techniques are usually laborious, time-consuming, often require complex costly instalments that inaccessible most laboratories. Synthetic nanoparticles face similar challenges, relying material-specific. In work, we introduce versatile micro-preparative (MP) method based polyacrylamide gel electrophoresis (PAGE) samples containing proteins, nucleic acids, bioconjugates, as well synthetic graphene quantum dots (GQDs). Using conventional vertical slab PAGE, demonstrate extraction purified DNA, DNA-protein bioconjugates from their respective mixtures MP-PAGE. We apply system recover DNA ladder mixture yields up 90%, compared 58% yield obtained specialized commercial devices. also folded enhanced yellow fluorescence protein (EYFP) crude cell extract 90% purity, comparable purities achieved two-step size exclusion immobilized metal-ion affinity chromatography procedure. Moreover, successful isolation an EYFP-DNA bioconjugate otherwise could not be processed Finally, technique was further extended size-dependent separation GQDs into three different fractions distinct optical properties. MP-PAGE thus offers rapid means purifying nanomaterials without for equipment.
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ژورنال
عنوان ژورنال: Meeting abstracts
سال: 2022
ISSN: ['2151-2043', '2152-8365', '2151-2035', '1091-8213']
DOI: https://doi.org/10.1149/ma2022-0112849mtgabs